EGFL6 regulates angiogenesis and osteogenesis in distraction osteogenesis via Wnt/β-catenin signaling
نویسندگان
چکیده
Abstract Background Osteogenesis is tightly coupled with angiogenesis during bone repair and regeneration. However, the underlying mechanisms linking these processes remain largely undefined. The present study aimed to test hypothesis that epidermal growth factor-like domain-containing protein 6 (EGFL6), an angiogenic factor, also functions in marrow mesenchymal stem cells (BMSCs), playing a key role interaction between osteogenesis angiogenesis. Methods We evaluated how EGFL6 affects activity of human umbilical cord vein endothelial (HUVECs) via proliferation, transwell migration, wound healing, tube-formation assays. Alkaline phosphatase (ALP) Alizarin Red S (AR-S) were used assay osteogenic potential BMSCs. qRT-PCR, western blotting, immunocytochemistry evaluate angio- osteo-specific markers pathway-related genes proteins. In order determine vivo, was injected into fracture gaps rat tibia distraction (DO) model. Radiography, histology, histomorphometry quantitatively osteogenesis. Results stimulated both differentiation through Wnt/β-catenin signaling vitro. Administration DO model promoted CD31 hi EMCN type H-positive capillary formation associated enhanced formation. Type H vessels referred subtype involved by EGFL6. Conclusion BMSCs accelerated regeneration stimulating Thus, increasing secretion appeared underpin therapeutic benefit promoting angiogenesis-coupled These results imply boosting local concentrations may represent new strategy for treatment compromised healing defect restoration.
منابع مشابه
Angiogenesis and mineralization during distraction osteogenesis.
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ژورنال
عنوان ژورنال: Stem Cell Research & Therapy
سال: 2021
ISSN: ['1757-6512']
DOI: https://doi.org/10.1186/s13287-021-02487-3